SCIENTIST

Job Description: We are seeking a highly skilled and experienced Senior Scientist with a proven track record in protein expression and purification within the industrial setting, preferably in a contract research organization. The ideal candidate will possess a strong background in biotechnology, biology, or a related field, along with extensive hands-on experience in protein purification techniques. Key Responsibilities: Lead and execute protein expression and purification projects according to client specifications and timelines. Utilize expertise in protein expression systems to optimize production yields and quality. Perform protein purification using AKTA FPLC systems and Unicorn control software, with proficiency in automating standard purification processes. Develop and validate purification methods to meet project requirements, ensuring high purity and yield of target proteins. Conduct analytical characterization of purified proteins using various techniques such as SDS-PAGE, Western blotting, and HPLC. Implement aseptic techniques for handling mammalian cell cultures, ensuring sterility and integrity throughout the purification process. A thorough understanding of bacterial endotoxin limits and strategies for their elimination from purified proteins. Collaborate effectively with cross-functional teams to troubleshoot and solve technical challenges. Mentor junior team members, providing guidance and support to foster their professional development. Contribute to the continuous improvement of laboratory protocols, processes, and workflows to enhance efficiency and quality. Qualifications: Master's degree (MSc. or M.Tech) in biotechnology, biology, or a related field. Ph.D. not required. 7 to 9 years of hands-on experience in protein expression and purification within an industrial setting, preferably in a contract research organization. Proficiency in protein expression systems such as bacterial, yeast, and mammalian cell cultures. Extensive experience with AKTA FPLC systems and Unicorn control software, including the ability to automate standard purification processes. Strong understanding of protein purification techniques and analytical methods. Familiarity with bacterial endotoxin limits and strategies for endotoxin removal. Excellent communication skills and ability to work collaboratively in a dynamic team environment. Demonstrated ability of proactive and problem-solving mindset. Ability to effectively prioritize and manage multiple projects simultaneously while meeting deadlines.